|
|
##xTract
|
|
|
###Targeted extraction of ion chromatograms.
|
|
|
|
|
|
The software xTract[1] has been designed to extract ion chromatograms of cross-linked peptides using the xQuest/xProphet[2-4] pipeline. It uses a unique target - decoy strategy to provide statistics on the extracted ion chromatograms. For this purpose, the software mProphet[5] is used.
|
|
|
|
|
|
![image](uploads/bf24fb00f31321b6d12e277e06dbd3a4/image.png)
|
|
|
|
|
|
###xTract example chromatogram: Shown are the extracted isotopes and the summed isotopes (cyan).
|
|
|
|
|
|
The software xTract consists of multiple programs that are executed consequtively.
|
|
|
All necessary programs are located in the /bin directory and have a help section (execute the program with the -h flag).
|
|
|
* xtract_add_precursor_intensity.pl, extracts the precursor intensities from the MS2 headers and adds it to the input ID file.
|
|
|
* xtract_prep.pl, prepares the folderstructures for the ion chromatogram extraction.
|
|
|
* xtract_decoys.pl, creates decoy peptides based on the target IDs.
|
|
|
* xtract_run.pl, executes the xtract.pl program.
|
|
|
* xtract_merger.pl, merges the results and runs the statistical analysis of peak groups by mProphet[4].
|
|
|
* xtract_normalizer.pl, generates normalization parameters using common identified peptides.
|
|
|
* xtract_analyzer.pl, analyzes the extraction results and provides statistics.
|
|
|
|
|
|
###References
|
|
|
1. Walzthoeni T, Joachimiak LA, Rosenberger G, Röst HL, Malmström L, Leitner A, Frydman R, Aebersold R (2015) xTract: software for characterizing conformational changes of protein complexes by quantitative cross-linking mass spectrometry. Nature methods.
|
|
|
2. Walzthoeni T, Claassen M, Leitner A, Herzog F, Bohn S, Förster F, Beck M, Aebersold R (2012) False discovery rate estimation for cross-linked peptides identified by mass spectrometry. Nature methods.
|
|
|
3. Rinner O, Seebacher J, Walzthoeni T, Mueller L, Beck M, Schmidt A, Mueller M, Aebersold R (2008) Identification of cross-linked peptides from large sequence databases. Nature methods.
|
|
|
4. Leitner A, Walzthoeni T, Aebersold R. (2014) Lysine-specific chemical cross-linking of protein complexes and identification of cross-linking sites using LC-MS/MS and the xQuest/xProphet software pipeline. Nature protocols.
|
|
|
5. Reiter et al. (2011) mProphet: automated data processing and statistical validation for large-scale SRM experiments. Nature methods. |