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The software xTract[1] has been designed to extract ion chromatograms of cross-linked peptides using the xQuest/xProphet[2-4] pipeline. It uses a unique target - decoy strategy to provide statistics on the extracted ion chromatograms. For this purpose, the software mProphet[5] is used.
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![image](uploads/bf24fb00f31321b6d12e277e06dbd3a4/image.png)
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xTract example chromatogram: Shown are the extracted isotopes and the summed isotopes (cyan).
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### xTract example chromatogram: Shown are the extracted isotopes and the summed isotopes (cyan).
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### Software components
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The software xTract consists of multiple programs that are executed consequtively.
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All necessary programs are located in the /bin directory and have a help section (execute the program with the -h flag).
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* xtract_add_precursor_intensity.pl, extracts the precursor intensities from the MS2 headers and adds it to the input ID file.
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